Thermo Fisher Scientific Rb Monoclonal Antibody (7E6)
Rb 단백질을 인식하는 마우스 단클론 항체로, Western blot, ELISA, IP에 사용 가능. 인간 재조합 단백질로부터 유래된 면역원 사용. U-2OS 세포를 양성 대조군으로 권장. 종양 억제 단백질 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5 µg/mL |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | 2 µg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7E6 |
| Immunogen | Recombinant human protein purified from E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_1960340 |
Product Specific Information
A suggested positive control for this product is U-2OS cells.
Target Information
Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms complexes with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription, and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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