Thermo Fisher Scientific NUP50 Polyclonal Antibody
NUP50 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, IP 등 다양한 응용에 사용 가능. 1 mg/mL 액상 형태로 제공되며 4°C에서 보관. 인간 NUP50(225-275 aa) 영역을 면역원으로 제작됨. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 225 and 275 of human nucleoporin 50 kDa (NUP50, UniProt ID: Q9UKX7-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4 °C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- Application note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
The nuclear pore complex is a large structure that spans the nuclear envelope, regulating the exchange of macromolecules between the nucleus and cytoplasm. Nucleoporins, including NUP50, are essential components of this complex. NUP50 is an FG-repeat containing nucleoporin that acts as a soluble cofactor in importin-α:β–mediated nuclear protein import. Pseudogenes are located on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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