Thermo Fisher Scientific XPA Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:200–1:1,000
• Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• For IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

The XPA (xeroderma pigmentosum group A) protein specifically recognizes UV- or chemically damaged DNA lesions and triggers the nucleotide excision repair process.
XPA binds to replication protein A (RPA) or excision repair cross complementing 1 protein (ERCC1).
In the absence of nucleotide excision repair, persisting DNA lesions may lead to gene mutations and ultimately to cancer.
Xeroderma pigmentosum patients have a >2000-fold increased risk of developing skin cancer at sun-exposed areas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.