Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), NovaFluor Blue 610-70S, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510, 585, 660-120S, 690
• NovaFluor Red 710, 755
• NovaFluor Yellow 570, 660, 730, 755
• PE, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Custom conjugation available upon request

Product Specific Information

• The 28-14-8 monoclonal antibody reacts with mouse MHC class I H-2D^b and cross-reacts with H-2L^d, H-2D^q, and/or H-2L^q.
• Binding to the alpha 3 domain of H-2L^d is independent of beta2-microglobulin.
• Each product contains one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.
• Reported for use in flow cytometric analysis.
• Tested using mouse splenocytes at ≤1 µg/test (100 µL final volume).
• Optimize antibody titration for best results.
• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.

CellBlox Plus Blocking Buffer Usage

• Required for optimal staining with NovaFluor conjugates.
• Add 5 µL per sample before combining antibody cocktails with cells.
• For single-color controls, use 5 µL per 100 µL sample (10³–10⁸ cells).
• Product ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01).

NovaFluor Conjugate Characteristics

• Based on Phiton™ technology utilizing nucleic acid dye structures.
• Engineered for optimized fluorescent signatures with reduced spillover and spread.
• Excitation: 509 nm
• Emission: 614 nm
• Laser: 488 nm (Blue)

Target Information

• MHC class I alloantigen involved in antigen presentation to CD3/TCR and CD8 T cells.
• Plays a key role in immunopeptidomics for neoantigen identification via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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