Thermo Fisher Scientific SSEA1 Monoclonal Antibody (MEM-158), PE
CD15(SSEA-1) 항원을 인식하는 Mouse IgM 단클론 항체로, PE 형광체가 결합되어 세포 표면 발현 분석에 적합. 인간 시료 반응성, Flow Cytometry 검증 완료. 4°C 암소 보관, 동결 금지.
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Applications
- Flow Cytometry (Flow): 20 µL per 100 µL whole blood or 10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-158 |
| Immunogen | Human granulocytes |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Purification | Size-exclusion chromatography |
| Storage Buffer | TBS, pH 8, with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1955091 |
Additional Formats
- Unconjugated (MA1-19094)
- APC (MA1-10106)
- Biotin (MA1-19490)
- FITC (MA1-19562)
- Request custom conjugation available
Product Specific Information
This antibody reacts with CD15, a cell membrane molecule (3-fucosyl-N-acetyllactosamine, 3-FAL) strongly expressed on the surface of granulocytes, monocytes, macrophages, and mast cells. It is also present on Langerhans cells and certain myeloid precursor cells.
This antibody does not cross-react with porcine, bovine, or sheep.
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of various cell types such as granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X. Cells with high Le(x) surface expression show strong self-aggregation via calcium-dependent Le(x)-Le(x) interaction, relevant in embryo compaction and teratocarcinoma cell aggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands. CD15 expression is widely used for Hodgkin’s disease diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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