Thermo Fisher Scientific E-cadherin Polyclonal Antibody
Rabbit polyclonal antibody recognizing human E-cadherin, validated for WB and Flow Cytometry. Detects N-terminal region (aa 160–189) of CDH1. Supplied as liquid, 1.5 mg/mL, in PBS with sodium azide. Suitable for cancer and cell adhesion research.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Flow Cytometry (Flow) | 1:10–1:50 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to amino acids 160–189 of the N-terminal region of human E-cadherin (CDH1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Purification | Ammonium sulfate precipitation, Size-exclusion, Dialysis |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2260238 |
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin protein belonging to the calcium-dependent adhesion protein superfamily. It is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail.
Mutations in the CDH1 gene, which encodes E-cadherin, are associated with gastric, breast, colorectal, thyroid, and ovarian cancers. Loss of E-cadherin function is linked to increased tumor proliferation, invasion, and metastasis.
E-cadherin also mediates bacterial adhesion to mammalian cells through its ectodomain, while the cytoplasmic domain is essential for internalization. The protein plays a central role in maintaining tissue architecture and integrity. In humans, the CDH1 gene is located on chromosome 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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