Thermo Fisher Scientific MIA Human ProcartaPlex Simplex Kit

Invitrogen™ MIA Human ProcartaPlex™ Simplex Kit

The Human MIA Simplex ProcartaPlex Kit measures MIA protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel utilizing Luminex xMAP technology for protein detection and quantitation. When combining multiple Simplex kits (e.g., when not using a pre-configured multiplex panel), only one buffer kit (Cat. No. EPX010-10420-901) is needed for each assay plate regardless of plex size.

Recommended sample pre-dilution for serum and plasma samples: 1:100

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as:

• 25 μL of plasma or serum
• 50 μL of cell culture supernatant
• Approximately 4 hours for analyzed results

Key Features:

• Reproducible, reliable results — validated to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample — measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology — the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures identifiable by Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. Each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). This bead-based system enables analysis of multiple targets in a single well.

The major difference between a ProcartaPlex assay and ELISA is that the capture antibody is conjugated to a magnetic bead rather than adsorbed to the microplate well, enabling free-floating reagents in solution. Detection is performed using Luminex instruments with dual lasers—one for bead identification and one for quantifying RPE fluorescence proportional to protein concentration. ProcartaPlex multiplex assays allow profiling of more target proteins using significantly less sample in the same time as a traditional ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine).
For more information, visit thermofisher.com/procartaplex.

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