Thermo Fisher Scientific CEA (CEACAM-5) Human ProcartaPlex Simplex Kit

Invitrogen™ CEA (CEACAM-5) Human ProcartaPlex™ Simplex Kit

The Human CEA (CEACAM-5) Simplex ProcartaPlex Kit measures CEA (CEACAM-5) protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel utilizing Luminex xMAP technology for protein detection and quantitation.
When combining multiple Simplex kits, only one buffer kit (Cat. No. EPX010-10420-901) is required per assay plate regardless of plex size.

Recommended sample pre-dilution for serum and plasma samples: 1:100

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument.
ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.

Key Features:

• Reproducible, reliable results — validated to the highest industry standard, including combinability and cross-reactivity testing
• More results per sample — measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology — the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures identified by Luminex xMAP systems (Luminex 200, FLEXMAP 3D, and MAGPIX).
Similar to a sandwich ELISA, the assay uses matched antibody pairs to identify the protein of interest. Each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE).
This conjugation allows analysis of multiple targets in a single well.

The major difference between ProcartaPlex and traditional ELISA is that the capture antibody is conjugated to a magnetic bead rather than adsorbed to the microplate well, enabling free-floating reagents in solution.
Detection instruments such as the Luminex 200 use dual lasers — one to distinguish bead signatures and another to quantify RPE fluorescence, proportional to protein concentration.
ProcartaPlex multiplex assays can profile more targets using significantly less sample in the same time as a traditional ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine).
For more information, visit thermofisher.com/procartaplex.

Specifications