Thermo Fisher Scientific Pyruvate Carboxylase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Pyruvate Carboxylase. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Pyruvate Carboxylase,uniProtId:P11498-1,ncbiNodeId:9606,antigenRange:1178,antigenLength:1178,antigenImageFileName:PA5-85705_Pyruvate_Carboxylase_P11498-1_Rabbit.svg,antigenImageFileNamePDP:PA5-85705_Pyruvate_Carboxylase_P11498-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.08 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2792844

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Rat (100%), Pig (100%), Rhesus Monkey (100%), Bovine (100%).

Positive Control: 293T, A431, HeLa, mouse liver.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Pyruvate Carboxylase requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.