Thermo Fisher Scientific RANK (soluble) Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MQIAPPCTSE KHYEHLGRCC NKCEPGKYMS SKCTTTSDSV CLPCGPDEYL DSWNEEDKCL LHKVCDTGKA LVAVVAGNST TPRRCACTAG YHWSQDCECC RRNTECAPGL GAQHPLQLNK DTVCKPCLAG YFSDAFSSTD KCRPWTNCTF LGKRVEHHGT EKSDAVCSSS LPARK.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sRANK Receptor. The anti-Human sRANK Receptor-specific antibody was purified by affinity chromatography using an immobilized Human sRANK Receptor matrix.

Sandwich ELISA:
To detect Human sRANK Receptor by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, used with PeproTech Biotinylated Anti-Human sRANK Receptor (500-P144Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human sRANK Receptor.

Western Blot:
To detect Human sRANK Receptor by Western Blot, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human sRANK Receptor is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

RANK is a member of the tumor necrosis factor receptor (TNFR) family. The full-length mouse RANK cDNA encodes a type I transmembrane protein with extracellular and cytoplasmic domains. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other TNFR family members in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.