Thermo Fisher Scientific COX-2 Polyclonal Antibody
COX-2 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, IP 등 다양한 응용에 적합합니다. Human에 반응하며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 항원 친화 크로마토그래피로 정제되었으며, 4°C에서 보관합니다.
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Thermo Fisher Scientific COX-2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 554 and 604 of human Cyclooxygenase-2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Sheep and Bovine
Target Information
COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins.
The conversion of arachidonate to prostaglandin H2 is a two-step reaction:
- Cyclooxygenase (COX) reaction: converts arachidonate to prostaglandin G2 (PGG2)
- Peroxidase reaction: reduces PGG2 to prostaglandin H2 (PGH2)
COX2 is constitutively expressed in some tissues under physiological conditions (endothelium, kidney, brain) and up-regulated under pathological conditions such as cancer and inflammation.
Up-regulation of COX2 is associated with increased cell adhesion, phenotypic changes, resistance to apoptosis, and tumor angiogenesis.
In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which modulates motility, proliferation, and resistance to apoptosis.
COX2 is naturally inhibited by calcitriol (active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo by interfering with transcription initiation and elongation.
COX2 is a target of NSAIDs such as aspirin, which reduce pain and swelling from inflammation driven by COX2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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