Thermo Fisher Scientific COX-2 Polyclonal Antibody

Thermo Fisher Scientific COX-2 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
• Based on 100% sequence identity, this antibody is predicted to react with Sheep and Bovine

Target Information

COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins.
The conversion of arachidonate to prostaglandin H2 is a two-step reaction:

1. Cyclooxygenase (COX) reaction: converts arachidonate to prostaglandin G2 (PGG2)
2. Peroxidase reaction: reduces PGG2 to prostaglandin H2 (PGH2)

COX2 is constitutively expressed in some tissues under physiological conditions (endothelium, kidney, brain) and up-regulated under pathological conditions such as cancer and inflammation.
Up-regulation of COX2 is associated with increased cell adhesion, phenotypic changes, resistance to apoptosis, and tumor angiogenesis.
In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which modulates motility, proliferation, and resistance to apoptosis.
COX2 is naturally inhibited by calcitriol (active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo by interfering with transcription initiation and elongation.
COX2 is a target of NSAIDs such as aspirin, which reduce pain and swelling from inflammation driven by COX2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.