Thermo Fisher Scientific COX-2 Polyclonal Antibody
COX-2 단백질을 인식하는 Rabbit Polyclonal Antibody로, Human 시료에 반응합니다. Western blot, IHC, IP 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 4°C에서 보관하며, 연구용으로만 사용됩니다.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific COX-2 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 554 and 604 of human Cyclooxygenase-2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Sheep and Bovine.
Target Information
COX2 converts arachidonate to prostaglandin H2 (PGH2), a key step in prostanoid synthesis and inflammatory prostaglandin production.
This process involves two reactions:
- Cyclooxygenase (COX) converts arachidonate to prostaglandin G2 (PGG2).
- Peroxidase reduces PGG2 to PGH2.
COX2 is constitutively expressed in tissues such as endothelium, kidney, and brain, and is upregulated under pathological conditions like cancer and inflammation.
Up-regulation is associated with increased cell adhesion, phenotypic changes, apoptosis resistance, and tumor angiogenesis.
In cancer cells, COX2 facilitates prostaglandin E2 (PGE2) production, influencing motility, proliferation, and apoptosis resistance.
COX2 activity is naturally inhibited by calcitriol (active Vitamin D) and suppressed by glucocorticoids.
It is a known target of NSAIDs such as aspirin, which reduce inflammation and pain.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.