Thermo Fisher Scientific USP15 Polyclonal Antibody
Thermo Fisher Scientific의 USP15 Polyclonal Antibody는 인간 및 마우스 샘플에 반응하는 Rabbit IgG 항체입니다. Western blot, ICC/IF, ELISA에 적합하며, 친화 크로마토그래피로 정제되었습니다. PBS/glycerol 용액 형태로 제공되며, -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 852–952 of human USP15 (NP_006304.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.53 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806676 |
Product Specific Information
- Immunogen sequence: SNHYGGMGGG HYTAFAKNKD DGKWYYFDDS SVSTASEDQI VSKAAYVLFY QRQDTFSGTG FFPLDRETKG ASAATGIPLE SDEDSNDNDN DIENENCMHT N
- Positive Samples: 293T, LO2
- Cellular Location: Cytoplasm, Nucleus
Target Information
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26S proteasome are coupled to multiple copies of ubiquitin. Attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity.
Deubiquitination is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families:
- Ubiquitin C-terminal hydrolases
- Ubiquitin-specific processing proteases (USPs)
- OTU-domain ubiquitin-aldehyde-binding proteins
- Jab1/Pad1/MPN-domain-containing metallo-enzymes
Among these, USPs are the most widespread deubiquitinating enzymes across evolution. They release ubiquitin from conjugated proteins and contain conserved catalytic domains with Cys and His boxes, and variable N- or C-terminal extensions for substrate recognition and localization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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