Thermo Fisher Scientific ANAPC11 Monoclonal Antibody (OTI1F2), TrueMAB
인간 ANAPC11 단백질을 표적으로 하는 Mouse IgG1 단클론 항체. Western blot 및 IHC(P)에서 검증됨. 동결건조 형태로 제공되며, 재구성 및 결합 실험에 적합. 세포주기 조절 관련 연구용으로 사용.
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Thermo Fisher Scientific ANAPC11 Monoclonal Antibody (OTI1F2), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F2 |
| Immunogen | Full length human recombinant protein of human ANAPC11 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of ~1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Together with the cullin protein ANAPC2, ANAPC11 forms the catalytic component of the anaphase promoting complex/cyclosome (APC/C) — a cell cycle-regulated E3 ubiquitin ligase controlling progression through mitosis and G1 phase.
The APC/C complex mediates ubiquitination and degradation of target proteins, primarily forming Lys-11-linked polyubiquitin chains, and to a lesser extent Lys-48 and Lys-63 chains.
May recruit E2 ubiquitin-conjugating enzymes to the complex.
[Source: UniProt]
Notice
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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