Thermo Fisher Scientific CD74 Monoclonal Antibody (1B8), APC

Applications and Tested Dilution

Product Specifications

Additional Formats

• FITC (Cat. No. MA5-45379)
• PerCP (Cat. No. MA5-45380)
• Custom conjugation available upon request

Product Specific Information

A 1:1,000 dilution of MA5-45378 was sufficient for detection of CD74 in 15 µg of B-cell lymphoma cell line Raji by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody. Detects approximately 33 kDa protein.

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons: exon 1 encodes the leader peptide, exons 2 and 3 encode the extracellular domains, and exon 4 encodes the transmembrane and cytoplasmic regions. DRA acts as the sole alpha chain for DRB1, DRB3, DRB4, and DRB5 without polymorphisms in the peptide-binding region. The beta chain contains all polymorphisms specifying peptide-binding specificity. HLA-DR is expressed on antigen-presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells, and activated T lymphocytes. The MHC class II molecules (DR, DQ, DP) bind intracellularly processed peptides and present them to T-helper cells, playing a crucial role in immune response initiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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