Thermo Fisher Scientific 6X His Tag Monoclonal Antibody (33D10.D2.G8), Cyanine5
6X His 태그 단백질 검출용 마우스 단클론 항체로 Cyanine5 형광 표지. ICC, Flow Cytometry, ELISA 등 다양한 응용에 적합. 높은 특이성과 낮은 비특이적 결합. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:1,000–1:5,000 |
| Flow Cytometry (Flow) | 1:500–1:2,500 |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 33D10.D2.G8 |
| Immunogen | HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide (H-H-H-H-H-H) conjugated to KLH using maleimide. |
| Conjugate | Cyanine5 |
| Excitation / Emission Max | 647 / 665 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Available Formats
- Unconjugated
- Atto 550
- Atto 647N
- Biotin
- Cyanine3
- DyLight 405, 488, 549, 649, 680, 800
- HRP
- Custom conjugation available
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
6X HIS Epitope Tag CY5 conjugated antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. It has reacted with all His-tagged proteins tested and shows no cross-reactivity with endogenous bacterial proteins. Recognizes His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at N- or C-terminal regions of recombinant proteins to facilitate purification by immobilized metal affinity chromatography (IMAC). Epitope tagging enables detection of recombinant proteins lacking specific antibodies. His-tagged proteins can be purified via Ni²⁺-NTA affinity chromatography due to strong and selective metal binding of histidine clusters. His tag-specific antibodies assist in detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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