Thermo Fisher Scientific TrxR1 Monoclonal Antibody (5A5)

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

1-2 µL

-

Product Specifications

Published species

Bovine

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5A5

Immunogen

Purified, recombinant, human thioredoxin reductase 1 protein expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2210231

Product Specific Information

A suggested positive control for this product is HeLa cells.

Target Information

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3` UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.