Thermo Fisher Scientific Ep-CAM/CD326 Recombinant Rabbit Monoclonal Antibody (EGP40, 1556R)
Ep-CAM/CD326 단백질을 인식하는 Recombinant Rabbit Monoclonal Antibody로, 인간 및 마우스 시료에 반응합니다. IHC, ICC/IF, Flow Cytometry에 적합하며, Protein A로 정제된 액상 형태입니다. Ep-CAM 발현 연구 및 종양 표지 분석에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | EGP40, 1556R |
| Immunogen | Recombinant human full-length TACSTD1 protein (EpCAM/CD326, UniProt ID: P16422-1, antigen range: 1–314) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- This antibody does not react with Mouse or Rat.
- For Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in epithelial cells (~40 kDa). It acts as a calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism through activation of c-myc and cyclin A/E genes.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression correlates with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial lineage tumors.
Ep-CAM is localized on the basolateral surface of most simple epithelia and carcinoma types and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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