Thermo Fisher Scientific CD3 Monoclonal Antibody (F7.2.38)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250-1:1,000

-

Immunohistochemistry (IHC)

1:100-1:250

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Product Specifications

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

F7.2.38

Immunogen

Purified CD3 epsilon gamma delta/CD3omega

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_10979571

Product Specific Information

MA5-12577 targets CD3E in IHC (P) applications and shows reactivity with Human samples.

The MA5-12577 immunogen is purified CD3 epsilon gamma delta/CD3omega.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.