Thermo Fisher Scientific Periostin (OSF-2) Human ProcartaPlex Simplex Kit

Invitrogen™ Periostin (OSF-2) Human ProcartaPlex™ Simplex Kit

The Human Periostin (OSF-2) Simplex ProcartaPlex Kit measures periostin (OSF-2) protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel utilizing Luminex xMAP technology for protein detection and quantitation.
When combining multiple Simplex kits (e.g., when not using a pre-configured multiplex panel), only one buffer kit (Cat. No. EPX010-10420-901) is needed for each assay plate regardless of plex size.

Recommended sample pre-dilution for serum and plasma samples: 1:100

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument.
ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.

• Reproducible, reliable results — validated to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample — measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology — the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures identifiable by Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX).
Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE).
The conjugation of protein-specific antibodies to distinct beads allows analysis of multiple targets in a single well.

The key difference between a ProcartaPlex assay and ELISA is that the capture antibody in ProcartaPlex is conjugated to a magnetic bead rather than adsorbed to the microplate well, allowing reagents to remain free-floating.
Detection via Luminex instruments employs two lasers—one to identify bead signatures and another to quantify RPE fluorescence proportional to protein concentration.
ProcartaPlex multiplex assays can profile more targets using less sample within the same time as a traditional ELISA.

ProcartaPlex multiplex panels are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine).
For more information, visit thermofisher.com/procartaplex.

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