Thermo Fisher Scientific AKT1 Monoclonal Antibody (G.145.7)
AKT1 단백질을 특이적으로 인식하는 Rabbit Monoclonal Antibody (Clone G.145.7). 인간, 마우스, 랫트 등 다양한 종에 반응. Western blot, IP, ChIP 등 응용 가능. Akt2 및 Akt3와 교차 반응 없음. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 3 publications |
| Immunoprecipitation (IP) | 1:50 | - |
| ChIP assay (ChIP) | 1–3 µL | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G.145.7 |
| Immunogen | Synthetic peptide surrounding Leu110 of human Akt1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 35 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10983391 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with Akt2 or Akt3.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells.
This protein kinase is activated by insulin, PI3K, IGF1, and various other growth and survival factors.
Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors and caspase-9.
The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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