Thermo Fisher Scientific EVI1 Monoclonal Antibody (S.446.4)
EVI1 단백질을 표적하는 Rabbit monoclonal antibody로, Western blot, ICC/IF, Flow cytometry, IP에 적합합니다. Affinity chromatography로 정제되었으며, -20°C에서 보관합니다. 인간 시료에 반응하며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:1,000–1:2,000 | - |
| Flow Cytometry (Flow) | 1:800–1:1,600 | - |
| Immunoprecipitation (IP) | 1:50 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S.446.4 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Asp57 of human Evi-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 651 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES (pH 7.5), 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10983870 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
EVI1 (MECOM) is a transcriptional regulator and oncoprotein involved in hematopoiesis, apoptosis, development, cell differentiation, and proliferation. It interacts with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. The EVI1 gene can undergo translocation with the AML1 gene, leading to EVI1 overexpression and leukemia onset. Several transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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