Thermo Fisher Scientific TCF7 Monoclonal Antibody (G.452.9)
TCF7 단백질을 인식하는 Rabbit Monoclonal Antibody(G.452.9)로, WB, ICC/IF, IP에 적합합니다. LEF1과 교차반응하지 않으며, 고순도의 액상 항체입니다. 인간, 마우스, 랫트 시료에 반응하며 -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G.452.9 |
| Immunogen | Synthetic peptide corresponding to a region surrounding Leu160 of human TCF1/TCF7 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 163 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10981895 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with LEF1.
Target Information
TCF7 (Transcriptional Factor 7) is a transcriptional activator involved in T-cell lymphocyte differentiation. It is necessary for the survival of CD4+ and CD8+ immature thymocytes. Isoforms of TCF7 lacking the N-terminal CTNNB1 binding domain cannot fulfill this role.
TCF7 binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. It may also act as a feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3, and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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