Thermo Fisher Scientific NUMB Monoclonal Antibody (OTI2E8), TrueMAB
NUMB 단백질을 표적하는 Mouse monoclonal antibody로, Western blot에 최적화되어 있습니다. Human 시료에 반응하며, Lyophilized 형태로 제공됩니다. 재구성 후 1 mg/mL 농도 사용 권장. 신경세포 분화 및 내재화 연구에 활용 가능합니다.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–365 of human NUMB produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Protein NUMB homolog is an evolutionarily conserved protein that regulates neuronal progenitor cell differentiation.
It functions in clathrin-dependent endocytosis by binding to the AP-2 complex and is expressed in postmitotic neurons where it supports axonal growth.
Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domains show extensive neurite outgrowth, which can be inhibited by VGCC inhibitors or Ca²⁺ chelators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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