Thermo Fisher Scientific GOLPH3 Polyclonal Antibody
GOLPH3 단백질을 표적으로 하는 Rabbit Polyclonal Antibody로, WB 및 ICC/IF에 적합합니다. Human, Mouse, Rat 반응성을 가지며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 1 mg/mL 농도이며, 2–8°C 보관 및 -20°C 장기 저장 가능합니다.
- 카탈로그번호
- PA520830
- 판매단위
- pk
카탈로그
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 20 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 14 amino acid peptide from near the amino terminus of human GOLPH3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154309 |
Product Specific Information
A suggested positive control is rat lung tissue lysate.
PA5-20830 can be used with blocking peptide PEP-0944.
Target Information
GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin.
GOLPH3 has since been implicated in the target of rapamycin (TOR) signaling pathway. Its overexpression in transfected cells led to an increase in anchorage-independent growth and cell proliferation in vitro.
Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating that GOLPH3 can enhance signaling through TOR-associated complexes.
These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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