Thermo Fisher Scientific Survivin Recombinant Rabbit Monoclonal Antibody (SP07-06)
Thermo Fisher Scientific의 Survivin Recombinant Rabbit Monoclonal Antibody (SP07-06)는 Western blot, IHC, ICC, Flow cytometry에 적합한 고품질 항체입니다. 인간 및 마우스 시료에 반응하며, HEK293 발현 시스템에서 생산된 recombinant monoclonal antibody입니다. 세포 내 Survivin 단백질 검출에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SP07-06 |
| Immunogen | Synthetic peptide within N-terminal human Survivin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2911561 |
Product Specific Information
- Positive Control: HeLa, human tonsil tissue, mouse testis tissue, human lymph node tissue
- Subcellular Location: Cytoplasm, Nucleus, Chromosome, Midbody
Target Information
Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. IAPs are involved in cell signaling, division, and metabolism, and are differentially expressed in most human cancers but not in normal tissues. Survivin encodes a protein with a single BIR domain and lacks a C-terminal RING finger domain.
Expression is high during fetal development and in tumors but low in adult tissues. Survivin is expressed in the G2/M phase of the cell cycle and associates with mitotic spindle microtubules. Disruption of this interaction increases caspase-3 activity and apoptosis during mitosis.
Survivin is also expressed in adult rat brain tissue following traumatic injury, co-localized with neuronal markers such as NeuN and PCNA, suggesting a role in neural cell proliferation. Multiple transcript variants exist, with at least three full-length isoforms identified. Overexpression in cancer may promote abnormal mitotic progression by inhibiting apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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