Thermo Fisher Scientific ROBO4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide made to a C-terminal region of the human protein (within residues 900-1007). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ROBO4,uniProtId:Q8WZ75-1,ncbiNodeId:9606,antigenRange:900-1007,antigenLength:1007,antigenImageFileName:PA1-46010_ROBO4_Q8WZ75-1_Rabbit.svg,antigenImageFileNamePDP:PA1-46010_ROBO4_Q8WZ75-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.1% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_2238660

Product Specific Information

In Western Blot, bands at 85 kDa and 107 kDa are observed, representing different isoforms of ROBO4 protein.

Suggested positive control: HUVEC lysate, antigen standard for ROBO4 (transient overexpression lysate).

Target Information

Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Involved in the maintenance of endothelial barrier organization and function (PubMed:30455415). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.