Thermo Fisher Scientific GATM Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.04–0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:1000–1:2500

Immunocytochemistry (ICC/IF)

• Tested Dilution: 0.25–2 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
LGRTLTGWVQ RTFQSTQAAT ASSRNSCAAD DKATEPLPKD CPVSSYNEWD PLEEVIVGRA ENACVPPFTI EVKANTYEKY WPFYQKQGGH YFPKDHLKKA VAEIEEMCNI LKTEGVTVRR PDPIDWSLKY KTPD

Highest antigen sequence identity to the following orthologs:

• Mouse: 93%
• Rat: 91%

Target Information

AGAT, also known as glycine amidinotransferase (L-arginine:glycine amidinotransferase), GATM or transamidinase, is a 423 amino acid protein belonging to the amidinotransferase family. Encoded by a gene that maps to human chromosome 15q21.1, AGAT exists as three alternatively spliced isoforms and consists of a homodimer, with equilibrium between monomeric and dimeric forms favoring a monomer subunit structure.
AGAT localizes to mitochondrial inner membranes, peripheral membranes, and cytoplasm. It is expressed in placenta, fetal tissues, brain, heart, liver, lung, salivary gland, and skeletal muscle, with high expression in kidney.
AGAT is elevated in myocardium during heart failure and decreased in IUGR-associated placenta. It catalyzes biosynthesis of guanidinoacetate, the immediate precursor of creatine, crucial for muscle energy metabolism.
AGAT defects are linked to arginine:glycine amidinotransferase deficiency, an autosomal recessive disorder causing developmental delay, mental retardation, and severe depletion of brain creatine/phosphocreatine.
AGAT may play roles in embryonic and CNS development and heart failure response via local creatine synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.