Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (eBioKAT-1 (KAT-1, KAT1)), APC, eBioscience
마우스 CD54(ICAM-1)을 인식하는 eBioKAT-1 단클론 항체로, APC 형광체가 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, 염증 반응 관련 연구에 유용합니다. 0.2 mg/mL 농도의 액상 형태로 제공되며, 4°C 암소 보관이 권장됩니다.
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Applications
Immunohistochemistry (IHC)
- Tested: Not specified
- View 1 publication
Flow Cytometry (Flow)
- Tested Dilution: 0.125 µg/Test
- View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioKAT-1 (KAT-1, KAT1) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762692 |
Additional Formats
Product Specific Information
The eBioKAT-1 monoclonal antibody reacts with mouse CD54 (ICAM-1), a 95 kDa member of the immunoglobulin superfamily. CD54 is expressed at low levels on leukocytes and high endothelial venules, with expression increasing in response to inflammatory cytokines. ICAM-1 binds to LFA-1, a key interaction required for T cell transendothelial migration. ICAM-1-deficient mice show reduced leukocyte migration to inflammation sites, leading to impaired immune responses.
This antibody recognizes a different epitope than the YN1/1.7.4 monoclonal antibody.
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes
- Recommended ≤ 0.125 µg per test (100 µL final volume)
- Cell number: 10⁵–10⁸ cells/test (optimize as needed)
- Excitation: 633–647 nm; Emission: 660 nm; Laser: Red
Target Information
ICAM-1 (CD54) is an 85–110 kDa single-chain type 1 integral membrane glycoprotein with five immunoglobulin-like extracellular domains, a transmembrane region, and a cytoplasmic domain. It contains seven potential N-linked glycosylation sites and shares sequence homology with ICAM-2 (CD102) and ICAM-3 (CD50). ICAM-1 binds to integrins CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1), and serves as a receptor for Rhinovirus.
ICAM-1 is expressed on activated endothelial cells, epithelial cells, fibroblasts, chondrocytes, B and T lymphocytes, monocytes, macrophages, dendritic cells, and neutrophils. Expression increases upon inflammation and is also observed in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in plasma and elevated in inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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