Thermo Fisher Scientific CD48 Monoclonal Antibody (HM48-1), APC, eBioscience

Thermo Fisher Scientific CD48 Monoclonal Antibody (HM48-1), APC, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.125 µg/test
  View 29 publications

Product Specifications

Available Formats

• Alexa Fluor 700
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 685
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The HM48-1 monoclonal antibody reacts with the mouse CD48 antigen, also known as BCM1, Blast-1 (human), and OX-45 (rat). CD48 is a 45 kDa GPI-linked glycoprotein expressed on most hematopoietic cells and plays a critical role in adhesion and T cell activation.

Members of the SLAM family, including CD48, CD150, and CD244, show differential expression among hematopoietic progenitors, providing a useful tool for stem cell characterization:

• HSCs: CD150(+)CD244(-)CD48(-)
• MPP: CD244(+)CD150(-)CD48(-)
• Restricted progenitors: CD48(+)CD244(+)CD150(-)

HM48-1 modulates CD48 functions in vitro and in vivo, including blocking CD48/CD2 and CD48/CD244 interactions, inhibiting spleen cell proliferation, providing costimulation for TCR-activated T cells, and prolonging cardiac allograft survival.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤ 0.125 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily, including CD2, CD58, CD84, CD150, CD229, and CD244. It is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a ligand for 2B4 and CD2.

Functions and expression:

• Strongly expressed on lymphocytes and monocytes
• Weakly on granulocytes
• Absent on platelets, fibroblasts, epithelium, and endothelium
• Marker for detecting GPI anchoring defects in PNH patients
• Involved in allergic airway disease pathology and potential therapeutic target for rhinitis and asthma

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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