Thermo Fisher Scientific Cas 9 Polyclonal Antibody, DyLight 800
DyLight 800 형광 표지된 Cas9 폴리클로날 항체로 Streptococcus pyogenes Cas9 단백질을 검출 및 정량화에 적합. Western blot, ICC/IF, ELISA 등에 사용 가능하며 높은 특이성과 안정성을 제공. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1 µg/mL
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1 µg/mL
ELISA
- Tested Dilution: 1:25,000–1:30,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein. |
| Conjugate | DyLight™ 800 |
| Excitation/Emission Max | 789/794 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- This product is stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9.
Target Information
Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering.
The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome.
Cas9 can also mediate upregulation of specific endogenous genes or alter histone modifications or DNA methylation.
Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has advantageous properties for genome editing, including small size, high efficiency, nickase activity, and apparent specificity.
SaCas9 recognizes NNGRRT PAM and cleaves target DNA at rates comparable to SpCas9 with spacer lengths of 24–20 nucleotides.
Compared to SpCas9, SaCas9 is smaller (1368 AA vs 1053 AA) and generates indels at a comparable rate, making it preferred for all-in-one delivery systems using adeno-associated vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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