Thermo Fisher Scientific Cas 9 Polyclonal Antibody, DyLight 800

Applications

Western Blot (WB)

• Tested Dilution: 1 µg/mL

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1 µg/mL

ELISA

• Tested Dilution: 1:25,000–1:30,000

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• This product is stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9.

Target Information

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering.
The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome.
Cas9 can also mediate upregulation of specific endogenous genes or alter histone modifications or DNA methylation.
Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has advantageous properties for genome editing, including small size, high efficiency, nickase activity, and apparent specificity.
SaCas9 recognizes NNGRRT PAM and cleaves target DNA at rates comparable to SpCas9 with spacer lengths of 24–20 nucleotides.
Compared to SpCas9, SaCas9 is smaller (1368 AA vs 1053 AA) and generates indels at a comparable rate, making it preferred for all-in-one delivery systems using adeno-associated vectors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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