Thermo Fisher Scientific ERK2 C-Term Polyclonal Antibody, DyLight 800
DyLight 800으로 표지된 ERK2 C-말단 폴리클로날 항체로, 인간, 마우스, 랫트 시료에 반응합니다. Western blot과 ELISA에 적합하며, ERK1과 교차 반응하지 않습니다. 안정성과 재현성이 우수하여 MAPK 신호전달 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-ERK2 antibody was produced in rabbits by repeated immunizations with a synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH. |
| Conjugate | DyLight™ 800 |
| Excitation / Emission Max | 789 / 794 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This DyLight™ 800 conjugated affinity purified antibody is directed against human ERK2 protein. Cross-reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK1 isoform.
Target Information
MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. MAP kinases (ERKs) act as integration points for multiple biochemical signals and are involved in various cellular processes such as proliferation, differentiation, transcription regulation, and development. Activation of this kinase requires phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of stimulated cells, where it phosphorylates nuclear targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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