Thermo Fisher Scientific Annexin A1 Monoclonal Antibody (M021)
인간 Annexin A1 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB, ICC/IF, IP, ELISA에 적합. 38 kDa Annexin A1 검출에 특이적이며 Annexin A2/A5는 인식하지 않음. 단백질 G 정제, PBS/BSA/glycerol buffer, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M021 |
| Immunogen | Clone M021 was generated from a proprietary antigen related to human Annexin A1 in the A549 lung carcinoma cell line. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942625 |
Product Specific Information
Clone M021 detects a 38 kDa protein corresponding to the molecular mass of Annexin A1 on SDS-PAGE immunoblots of human A431, MDA-MB-231, and A549 cells. The antibody also detects full-length recombinant human Annexin A1, but not Annexin A2 or Annexin A5 recombinant proteins.
This antibody is suitable for Western blot, ELISA, immunocytochemical labeling, and immunoprecipitation.
Target Information
Annexin I belongs to a family of Ca²⁺-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. Annexin I has a molecular mass of 40 kDa and exhibits phospholipase A2 inhibitory activity.
Since phospholipase A2 is required for the biosynthesis of potent mediators of inflammation such as prostaglandins and leukotrienes, Annexin I may have potential anti-inflammatory activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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