Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), NovaFluor Blue 610-30S, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.2 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV / Violet / Yellow Series
• FITC, PE, PE-Cyanine5, PE-Cyanine7
• Super Bright series
• NovaFluor Blue, Red, UV, Violet, Yellow series
• Functional Grade
• Custom conjugation available

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, subsets of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and plays a key role in T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes. Recommended ≤0.2 µg/test (in 100 µL final volume). Optimize antibody titration for best performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates.
• Add 5 µL of CellBlox Plus Blocking Buffer per sample before combining with cells.

NovaFluor Technology:
Based on Phiton™ technology utilizing novel nucleic acid dye structures engineered for optimized fluorescent signatures with minimal spillover and spread.

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and functions as a co-receptor for HIV. It is primarily expressed on T helper cells but also found on monocytes, macrophages, and dendritic cells. CD4 expression occurs in thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues. It plays a critical role in T-cell activation via association with the T-cell receptor complex and Lck kinase. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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