Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested dilution: 0.2 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV/Violet/Yellow series
• PE, PE-Cyanine5, PE-Cyanine7
• Super Bright series
• NovaFluor Blue/Red/UV/Violet/Yellow series
• Request custom conjugation available

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, a subset of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and is crucial for T cell development and function. Binding of GK1.5 is blocked by RM4-5.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.2 µg/test (100 µL sample; 10⁵–10⁸ cells/test). Optimize titration for best results.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails/master mixes before combining with cells.
  • For single-color controls: 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor Conjugates:

• Based on Phiton™ technology using novel nucleic acid dye structures.
• Designed for engineered fluorescent signatures minimizing spillover and spread.
• Excitation: 509 nm; Emission: 614 nm; Laser: 488 nm (Blue).

Target Information

CD4 antigen is involved in the recognition of MHC class II molecules and serves as a co-receptor for HIV. It is expressed primarily on T helper cells, as well as monocytes, macrophages, and dendritic cells. CD4 expression is observed in thymus, lymph nodes, tonsils, spleen, and some non-lymphoid tissues. It plays a key role in T-cell activation via interaction with Lck kinase and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts exist (RefSeq, July 2017).

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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