Thermo Fisher Scientific Ki-67 Recombinant Rabbit Monoclonal Antibody (BLR021E)
인간 Ki-67 단백질을 인식하는 Thermo Fisher의 재조합 토끼 단클론 항체입니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 사용 가능합니다. 세포 증식 마커로 연구용에 적합하며, 1 mg/mL 농도의 액상 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR021E |
| Immunogen | Peptide representing the repetitive 66 bp element of Human Ki-67 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
This product is tested in Multiplex Immunofluorescence (mIF).
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle—particularly in late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that allows association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and the nucleolus. Ki-67 shows cell cycle-dependent localization: perinucleolar expression at G1, nuclear matrix expression at G2, and chromosomal expression during M phase.
As a proliferation marker, Ki-67 is absent in G0 cells and increases from G1 through mitosis. Ki-67 antibodies are widely used to determine the growth fraction in neoplasms, showing strong correlation between low Ki-67 index and histologically low-grade tumors. It is routinely used as a neuronal marker of cell cycling and proliferation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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