Thermo Fisher Scientific Granzyme B Recombinant Rabbit Monoclonal Antibody (BLR022E)
Granzyme B를 표적으로 하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 적합합니다. HEK293 세포에서 발현되었으며 Protein A로 정제되었습니다. 인체 반응성이 있으며 액상 형태로 제공되어 연구용으로 사용됩니다.
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Thermo Fisher Scientific Granzyme B Recombinant Rabbit Monoclonal Antibody (BLR022E)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1 µL per 1 × 10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR022E |
| Immunogen | Recombinant protein representing human Granzyme B (residues 19–247) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 50 µg/mL |
| Purification | Protein A |
| Storage Buffer | BBS, pH 8.2, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note:
- For ICC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE cell sections.
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
Granzyme B is a member of the granzyme serine protease family found in the granules of cytotoxic T cells and NK cells. It induces apoptosis in target cells through caspase activation and mitochondrial pathways. Granzyme B interacts with the mannose 6-phosphate receptor, functioning as a death receptor during cytotoxic T cell-induced apoptosis. It plays a critical role in immune-mediated cell death and has been identified as CGL1 and CTLA-1 based on mRNA expression in activated cytotoxic T lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: A700-022_Granzyme_B_P10144-1_Rabbit.svg, A700-022_Granzyme_B_P10144-1_Rabbit_PDP.jpeg)
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