Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (1D11), Brilliant Ultra Violet 615, eBioscience

Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (1D11), Brilliant Ultra Violet 615, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested dilution: 5 µL (0.5 µg)/test

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Custom conjugation available

Product Specific Information

The 1D11 monoclonal antibody reacts with human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, γδ T cells, CD8+ T cells, and some CD4+ T cells. NKG2D forms complexes with DAP10, acting as a co-stimulatory receptor. Its counter-receptors are MICA/MICB, expressed on various epithelial tumors.

5C6 and 1D11 clones block soluble MICA binding to γδ TCR T cell clones and inhibit cell lysis, while also inducing NKG2D-mediated redirected lysis of Fc receptor-bearing P815 cells.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-diluted and validated on normal human peripheral blood cells. Use 5 µL (0.5 µg) per 100 µL cell sample (10⁵–10⁸ cells/test).

Brilliant Ultra Violet™ 615 (BUV615):
A tandem dye emitting at 615 nm, optimized for cytometers with a 355 nm UV laser. Confirm instrument compatibility before use.

Staining Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, use one of the following to reduce non-specific interactions:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Light Sensitivity:
Protect vial and stained samples from light due to photo-induced oxidation sensitivity.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)

Excitation: 350 nm; Emission: 615 nm; Laser: Ultraviolet.

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Natural killer (NK) cells mediate lysis of tumor and virus-infected cells without prior activation and regulate immune responses. The NKG2 gene family encodes C-type lectin-like receptors expressed in NK cells, located within the NK complex region.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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