Thermo Fisher Scientific iNOS Monoclonal Antibody (CXNFT), Brilliant Ultra Violet 615, eBioscience

Thermo Fisher Scientific iNOS Monoclonal Antibody (CXNFT), Brilliant Ultra Violet 615, eBioscience

Applications

• Reported Application: Intracellular staining followed by flow cytometric analysis
• Tested Application: Flow Cytometry (Flow)
• Tested Dilution: 0.125 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 786
• eFluor 450
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This CXNFT monoclonal antibody reacts to mouse NOS2 (inducible NOS, iNOS). Nitric oxide synthase enzymes catalyze the formation of nitric oxide from L-arginine through an NADPH- and oxygen-dependent mechanism.
There are three isoforms of NOS: NOS1 (nNOS), NOS3 (eNOS), and NOS2 (iNOS). NOS2 is induced by bacterial endotoxins and inflammatory cytokines such as IFN-γ and TNF-α. It is expressed by M1 macrophages and myeloid-derived suppressor cells but not by M2 macrophages.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on mouse thioglycolate-elicited peritoneal exudate cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended ≤ 0.125 µg per test.

Brilliant Ultra Violet™ 615 (BUV615) emits at 615 nm and is suitable for UV (355 nm) laser-equipped cytometers.
Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.

Light Sensitivity: Protect from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark.

Excitation: 350 nm
Emission: 615 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

iNOS (Inducible Nitric Oxide Synthase) produces nitric oxide (NO) from L-arginine, mediating vasorelaxation, neurotransmission, and cytotoxicity.
iNOS is inducible by cytokines (IFN-γ, TNF, IL-1, IL-2) and lipopolysaccharides (LPS). It is expressed in macrophages, hepatocytes, synoviocytes, and smooth muscle cells.
Three iNOS pseudogenes are located on chromosome 17 (Smith-Magenis syndrome region).
Diseases associated with iNOS dysfunction include achalasia and impotence.

For Research Use Only. Not for use in diagnostic procedures.

제품 이미지