Thermo Fisher Scientific KLRG1 Monoclonal Antibody (2F1), Brilliant Ultra Violet 615, eBioscience

Thermo Fisher Scientific KLRG1 Monoclonal Antibody (2F1), Brilliant Ultra Violet 615, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: -

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 647
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 563
• Brilliant Violet 421 / 480 / 605 / 711
• eFluor 450 / 506
• FITC
• Functional Grade
• Pacific Orange
• PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

This 2F1 monoclonal antibody reacts with mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer of glycosylated 30–38 kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM).

KLRG1 is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes; recommended ≤ 0.5 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Antibody titration recommended for optimal performance.

Fluorochrome Information:
Brilliant Ultra Violet™ 615 (BUV615) emits at 615 nm and is designed for use with ultraviolet (355 nm) laser-equipped cytometers.

Staining Buffer Recommendation:
Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) when using multiple polymer dye-conjugated antibodies to minimize non-specific interactions.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark.

Excitation: 350 nm
Emission: 615 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that interact with SH2 domains of phosphatases such as SHP-1.
KLRG1 is an inhibitory receptor expressed on NK cells and certain T cells, involved in tumor and virus-infected cell lysis and immune regulation.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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