Thermo Fisher Scientific USP7 Polyclonal Antibody
USP7(HAUSP) 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, Flow Cytometry에 사용 가능. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived human HAUSP/USP7 recombinant protein (Position: L258–D483) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2747324 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
USP7 (also known as HAUSP) is a ubiquitin-specific protease that removes ubiquitin from its substrates. It plays a crucial role in regulating the stability of cellular proteins, particularly by antagonizing Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein p53. By deubiquitinating p53, HAUSP stabilizes p53 and prevents its degradation, suggesting a tumor suppressor function in response to cellular stress. Additionally, HAUSP may provide an alternative pathway for protecting cells from oncogenic insults through ARF-dependent and ARF-independent mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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