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Thermo Fisher Scientific B7-H7 Monoclonal Antibody (MA57YW), PE-eFluor 610, eBioscience
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Thermo Fisher Scientific

Thermo Fisher Scientific B7-H7 Monoclonal Antibody (MA57YW), PE-eFluor 610, eBioscience

상품 한눈에 보기

인간 B7-H7 단백질을 인식하는 MA57YW 단일클론 항체로, PE-eFluor 610 형광체가 결합된 제품입니다. Flow cytometry 분석에 최적화되어 있으며, 488–561 nm 레이저로 여기되어 607 nm에서 방출됩니다. 고순도 정제 및 안정적 형광 신호 제공.

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Thermo Fisher Scientific
카테고리
Monoclonal Antibodies
판매단위
pk

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Thermo Fisher Scientific 61-6537-42 B7-H7 Monoclonal Antibody (MA57YW), PE-eFluor 610, eBioscience 100 tests pk판매 단위 pk
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Thermo Fisher Scientific · Thermo Fisher Scientific B7-H7 Monoclonal Antibody (MA57YW), PE-eFluor 610, eBioscience

Thermo Fisher Scientific B7-H7 Monoclonal Antibody (MA57YW), PE-eFluor 610, eBioscience

Applications and Tested Dilution

  • Application: Flow Cytometry (Flow)
  • Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

항목 내용
Species Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Recommended Isotype Control Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor™ 610, eBioscience™
Class Monoclonal
Type Antibody
Clone MA57YW
Immunogen B7-H7 recombinant protein
Conjugate PE-eFluor™ 610
Excitation/Emission Max 565 / 606 nm
Form Liquid
Concentration 5 µL/test
Purification Affinity chromatography
Storage Buffer PBS, pH 7.2, with 0.2% BSA
Contains 0.09% sodium azide
Storage Conditions 4°C, store in dark, DO NOT FREEZE
Shipping Conditions Ambient (domestic); Wet ice (international)
RRID AB_2784792

Additional Formats

Product Specific Information

This MA57YW monoclonal antibody recognizes human B7-H7 on live and paraformaldehyde-fixed cells.

  • Applications Reported: Flow cytometric analysis
  • Applications Tested: Pre-diluted and tested on HDLM-2 cell line
    • Recommended use: 5 µL (0.5 µg) per test
    • A test is defined as staining a cell sample in 100 µL final volume
    • Cell number range: 10⁵–10⁸ cells/test

Fluorochrome characteristics:
PE-eFluor 610 can be excited with laser lines from 488–561 nm and emits at 607 nm.
Recommended filter: 610/20 band pass (PE-Texas Red equivalent).
Ensure instrument compatibility with this fluorochrome.

Light sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples can be stored in:

Storage: up to 3 days at 4°C in dark with minimal impact on brightness and FRET efficiency.
Clone-specific performance should be empirically determined.

Excitation: 488–561 nm
Emission: 607 nm
Compatible Lasers: Blue, Green, Yellow-Green

Target Information

B7-H7 (also known as HHLA2) is a membrane protein with three Ig-like domains (IgV-IgC-IgV), unlike other B7 family members with two domains.
It shows 10–18% amino acid identity and 23–33% similarity to other B7 proteins.
B7-H7 is expressed in epithelial cells of gallbladder, breast, kidney, colon, small intestine, and placenta.
In the immune system, HHLA2 is not constitutively expressed on human monocytes/macrophages.
It is widely expressed in cancers, especially triple-negative breast cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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