Thermo Fisher Scientific B7-H7 Monoclonal Antibody (MA57YW), PE-eFluor 610, eBioscience
인간 B7-H7 단백질을 인식하는 MA57YW 단일클론 항체로, PE-eFluor 610 형광체가 결합된 제품입니다. Flow cytometry 분석에 최적화되어 있으며, 488–561 nm 레이저로 여기되어 607 nm에서 방출됩니다. 고순도 정제 및 안정적 형광 신호 제공.
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Thermo Fisher Scientific B7-H7 Monoclonal Antibody (MA57YW), PE-eFluor 610, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.5 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor™ 610, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MA57YW |
| Immunogen | B7-H7 recombinant protein |
| Conjugate | PE-eFluor™ 610 |
| Excitation/Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2784792 |
Additional Formats
Product Specific Information
This MA57YW monoclonal antibody recognizes human B7-H7 on live and paraformaldehyde-fixed cells.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-diluted and tested on HDLM-2 cell line
- Recommended use: 5 µL (0.5 µg) per test
- A test is defined as staining a cell sample in 100 µL final volume
- Cell number range: 10⁵–10⁸ cells/test
Fluorochrome characteristics:
PE-eFluor 610 can be excited with laser lines from 488–561 nm and emits at 607 nm.
Recommended filter: 610/20 band pass (PE-Texas Red equivalent).
Ensure instrument compatibility with this fluorochrome.
Light sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in:
- IC Fixation Buffer (Product # 00-8222-49)
- 1-step Fix/Lyse Solution (Product # 00-5333-57)
Storage: up to 3 days at 4°C in dark with minimal impact on brightness and FRET efficiency.
Clone-specific performance should be empirically determined.
Excitation: 488–561 nm
Emission: 607 nm
Compatible Lasers: Blue, Green, Yellow-Green
Target Information
B7-H7 (also known as HHLA2) is a membrane protein with three Ig-like domains (IgV-IgC-IgV), unlike other B7 family members with two domains.
It shows 10–18% amino acid identity and 23–33% similarity to other B7 proteins.
B7-H7 is expressed in epithelial cells of gallbladder, breast, kidney, colon, small intestine, and placenta.
In the immune system, HHLA2 is not constitutively expressed on human monocytes/macrophages.
It is widely expressed in cancers, especially triple-negative breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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