Thermo Fisher Scientific B7-H7 Monoclonal Antibody (MA57YW), PE-eFluor 610, eBioscience

Thermo Fisher Scientific B7-H7 Monoclonal Antibody (MA57YW), PE-eFluor 610, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• APC
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

This MA57YW monoclonal antibody recognizes human B7-H7 on live and paraformaldehyde-fixed cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on HDLM-2 cell line
  • Recommended use: 5 µL (0.5 µg) per test
  • A test is defined as staining a cell sample in 100 µL final volume
  • Cell number range: 10⁵–10⁸ cells/test

Fluorochrome characteristics:
PE-eFluor 610 can be excited with laser lines from 488–561 nm and emits at 607 nm.
Recommended filter: 610/20 band pass (PE-Texas Red equivalent).
Ensure instrument compatibility with this fluorochrome.

Light sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-57)

Storage: up to 3 days at 4°C in dark with minimal impact on brightness and FRET efficiency.
Clone-specific performance should be empirically determined.

Excitation: 488–561 nm
Emission: 607 nm
Compatible Lasers: Blue, Green, Yellow-Green

Target Information

B7-H7 (also known as HHLA2) is a membrane protein with three Ig-like domains (IgV-IgC-IgV), unlike other B7 family members with two domains.
It shows 10–18% amino acid identity and 23–33% similarity to other B7 proteins.
B7-H7 is expressed in epithelial cells of gallbladder, breast, kidney, colon, small intestine, and placenta.
In the immune system, HHLA2 is not constitutively expressed on human monocytes/macrophages.
It is widely expressed in cancers, especially triple-negative breast cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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