Thermo Fisher Scientific ROR gamma (t) Monoclonal Antibody (B2D), PE-eFluor 610, eBioscience
마우스 ROR gamma(t) 단백질을 인식하는 B2D 단클론 항체로, PE-eFluor 610 형광체가 결합되어 있습니다. Flow Cytometry 및 IHC(Frozen)에 적합하며, 0.25 µg/test로 권장됩니다. 4°C 암소 보관, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 13 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PE-eFluor™ 610, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B2D |
| Conjugate | PE-eFluor™ 610 |
| Excitation/Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574649 |
Additional Formats
Product Specific Information
The B2D monoclonal antibody reacts with mouse ROR gamma (t) protein, a member of the retinoic acid-related orphan receptor (ROR) family, which includes ROR alpha and ROR beta. These are ligand-dependent transcription factors involved in multiple physiological processes.
ROR gamma is expressed in tissues such as liver, lung, muscle, heart, and kidney. The isoform ROR gamma t is expressed specifically in lymphoid cells including CD4+CD8+ thymocytes, Th17 cells, and lymphoid tissue inducer (Lti) cells. The B2D antibody recognizes both ROR gamma and ROR gamma t isoforms.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested with mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00) and protocol. Recommended usage ≤ 0.25 µg/test (100 µL final volume).
Fluorophore Information:
PE-eFluor® 610 can be excited with 488–561 nm lasers and emits at 607 nm. Recommended filter: 610/20 band pass (PE-Texas Red® equivalent). Protect from light to prevent photo-induced oxidation.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark.
Excitation/Emission: 488–561 nm / 607 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
ROR gamma (NR1 Thyroid Hormone-Like receptor) influences thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. It promotes thymocyte survival by activating antiapoptotic protein Bcl-x(L) and is essential for lymph node and Peyer’s patch development.
The thymus-specific isoform ROR gamma(t) inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to DNA response elements with the GGTCA motif. Expression is documented in mouse thymus, adipose, bone, skeletal muscle, liver, kidney, and human skeletal muscle.
Note: For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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