Thermo Fisher Scientific Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE-eFluor 610, eBioscience

Thermo Fisher Scientific Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE-eFluor 610, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: View 82 publications

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Product Specifications

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Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 395
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 711
• Brilliant Violet 786
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

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Product Specific Information

The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins.
D7 binds both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid populations, and peripheral T and B cells.
Non-activated splenocytes from Ly-6.2 strains (C57BL, SJL, 129, AKR, etc.) show higher frequency of D7-positive cells compared to Ly-6.1 strains (BALB/c, C3H, NZB, etc.).
Sca-1 is expressed at high levels upon activation regardless of Ly-6 haplotype and is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subsets.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse bone marrow cells (≤0.5 µg/test).
A test is defined as the amount of antibody that stains a cell sample in 100 µL volume.
Cell number may vary (10⁵–10⁸ cells/test); antibody should be titrated for optimal performance.

Fluorophore Characteristics:

• PE-eFluor® 610 excitation: 488–561 nm
• Emission: 607 nm
• Recommended filter: 610/20 band pass (PE-Texas Red® equivalent)
• Compatible lasers: Blue, Green, Yellow-Green

Light Sensitivity: This tandem dye is sensitive to photo-induced oxidation; protect vial and samples from light.

Fixation: Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)
  Minimal impact on brightness and FRET efficiency/compensation. Clone-specific performance should be determined empirically.

Filtration: 0.2 µm post-manufacturing filtered.

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Target Information

Stem Cell Antigen-1 (Sca-1) is a member of the Ly-6 family, expressed on multipotent hematopoietic stem cells (HSC) and used as a marker of HSC in mice.
Sca-1 is used with lineage depletion antibodies to identify murine HSC and is found in fetal liver, adult bone marrow, peripheral blood, and spleen.
It may be involved in the regulation of B and T cell activation.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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