Thermo Fisher Scientific CD3/CD4 Antibody Cocktail, FITC, PE, eBioscience

Thermo Fisher Scientific CD3/CD4 Antibody Cocktail, FITC, PE

Applications

• Reported Use: Flow cytometric analysis
• Tested Use: Pre-titrated and validated for flow cytometry of normal human peripheral blood cells
• Recommended Dilution: 20 µL/test (contains 1 µg Anti-Human CD3 (UCHT1) FITC and 0.5 µg Anti-Human CD4 (RPA-T4) PE)
• One test corresponds to staining a cell sample in 100 µL final volume (10⁵–10⁸ cells/test)

Product Specifications

Product Specific Information

This product consists of two fluorochrome-conjugated monoclonal antibodies for distinguishing human peripheral CD3⁺ and CD4⁺ T cells.

• UCHT1 (Anti-CD3ε): Mouse IgG1, kappa antibody reacting with human CD3ε (20 kDa), part of the TCR complex essential for proper assembly and surface expression. Expressed on thymocytes and mature T cells.
• RPA-T4 (Anti-CD4): Mouse IgG1, kappa antibody binding human CD4 (59 kDa), a receptor expressed on thymocytes, T-helper cells, and monocytes. CD4 also functions as the HIV receptor.

Target Information

• CD3 Complex: Transduces antigen recognition signals in T cells and regulates TCR surface expression. Composed of γ, δ, ε, and ζ subunits encoded on chromosome 11.
• Functional Role: Mediates signal transduction via cytoplasmic tails containing tyrosine-based motifs.
• Expression: Found on 68–82% of peripheral lymphocytes and thymocytes; absent on B and NK cells.
• Clinical Relevance: Altered T cell percentages may be observed in autoimmune diseases.
• CD4 Antigen: Involved in MHC class II recognition and HIV binding; associated with p56lck tyrosine kinase; expressed in lymph nodes and tonsils.
• CD44 Reference: Type I transmembrane glycoprotein acting as a hyaluronate receptor, involved in leukocyte adhesion and migration.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.