Thermo Fisher Scientific NCOA4 Monoclonal Antibody (OTI2F1), TrueMAB
인간 NCOA4 단백질(aa 280-553)에 특이적인 Mouse IgG1 단일클론 항체. Western blot에 1:2,000 희석으로 검증됨. 동결건조 형태로 제공되며, PBS 및 trehalose 포함. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 280–553 of human NCOA4 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Androgen receptor (AR) coactivator ARA70, also designated NCOA4, RFG, and ELE1, is a co-activator that enhances androgen receptor activity.
In human thyroid carcinomas, the Ret proto-oncogene fuses to ARA70 to form Ret/PTC3 via intrachromosomal inversion.
ARA70 exists as two isoforms: ARA70a and ARA70b.
- ARA70a: Widely expressed; highest in testis and adipose tissues.
- ARA70b: Expressed only in testis.
ARA70a functions as a ligand-enhanced co-activator of PPARγ in adipocytes.
ARA70 may play roles in prostate and ovarian carcinogenesis and mediate estrogen-androgen synergism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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