Thermo Fisher Scientific CSF1 Monoclonal Antibody (OTI8E1), TrueMAB

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a critical survival factor for the proliferation and differentiation of monocytes, macrophages, and osteoclast progenitors. It also induces VEGF secretion by macrophages, promoting endothelial progenitor mobilization and neovascularization.

M-CSF exists as several bioactive isoforms differing in potency and stability. The full-length protein is membrane-associated and may be cleaved into secreted forms. It functions as a disulfide-bonded homodimer that can exist as either a glycoprotein or a proteoglycan modified with chondroitin sulfate.

Binding of M-CSF to its receptor c-Fms (CSF-1R or CD115) triggers receptor dimerization, internalization, and degradation. Functionally, M-CSF stimulates differentiation of hematopoietic stem cells into monocyte-macrophage populations in culture.
Note: Human M-CSF is active on mouse cells, but mouse CSF shows no activity on human cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.