Thermo Fisher Scientific IRAK1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

2-4 µg

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide: C-terminus of human IRAK

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_1958259

Product Specific Information

Recommended positive controls: THP-1 or HeLa whole cell lysate.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. It is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. IRAK`s association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Alternatively, IRAK1 can phosphorylate TIRAP to promote its ubiquitination and subsequent degradation. IRAK1 also phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus. This results in the transcriptional activiation of type I IFN genes, which drive the cell in an antiviral state.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.