Thermo Fisher Scientific GATA-3 (Center) (Breast and Urothelial Marker) Monoclonal Antibody (GATA3/6664)
GATA-3 단백질을 인식하는 단클론 항체로, 유방 및 요로상피 종양 마커 연구에 적합. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. Human 반응성, Mouse IgG2b 형, 액상 형태로 제공. -20°C에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2 µg/mL |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GATA3/6664 |
| Immunogen | Recombinant full-length human GATA-3 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Target Information
The genes for all four subunits of the T-cell antigen receptor (alpha, beta, gamma, and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all four TCR genes.
GATA3, shown in chicken studies to contain a zinc finger domain, is abundantly expressed in T-lymphocytes and participates in T-cell receptor gene activation through enhancer binding. Labastie et al. (1994) cloned the human gene, which comprises six exons over 17 kb of DNA, with two zinc fingers encoded by conserved exons similar to GATA1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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