Thermo Fisher Scientific GATA-3 (Breast and Urothelial Marker) Recombinant Mouse Monoclonal Antibody (rGATA3/9133)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• Positive Control: Human breast or urothelial carcinoma tissue
• Cellular Location: Nucleus

Target Information

The genes for all four subunits of the T-cell antigen receptor (alpha, beta, gamma, and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all four TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises six exons distributed over 17 kb of DNA. Its two zinc fingers are encoded by two separate exons highly conserved with those of GATA1, but no other structural homologies between the two genes could be found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.